Mulch removal time did not have significant effects on Tuber melanosporum mycelium biomass

Aim of study: We aimed to i) evaluate the effects mulching on Tuber melanosporum mycelium biomass and seedling growth (i.e. root collar diameter height) ii) unravel relationship between in abundance, a T. plantation.Area The experimental plantation is located Pre-Pyrenees mountains Catalonia, Spain.Material methods: was established 2010 using one-year-old inoculated Quercus ilex seedlings. Double-layered mulch materials were placed around removed from randomly selected seedlings 2015 2018. Soil samples collected 2018 at 40 80 cm distances that had during five eight years, estimated by quantitative Polymerase Chain Reaction (qPCR). Seedling height measured simultaneously when removed.Main results: Mulch removal time did not have significant or growth. However, distance tended be higher after eight-year with 0.9 mg/g soil whereas 0.4 five-year mulching. A positive also found.Research highlights: Mulching seems effect truffle biomass, nearly two times quantity eight-years compared five-years usage. good indicator expansion plantation.Keywords: Black truffle; ilex; mulching; tree growth; cultivation.